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Molecular cytogenetic characterization and comparison of the two cultivated Canavalia species (Fabaceae)

by: She, Chao-Wen; Wei, Lin; Jiang, Xiang-Hui

Publication date: 2017-9-12
Usage: Attribution 4.0 International
Topics: Canavalia cultivars, karyotype, 5S rDNA, 45S rDNA, fluorochrome banding, in situ hybridization
Publisher: Pensoft Publishers
Collection: biodiversity
Contributor: Pensoft Publishers
Language: English
Rights: https://biodiversitylibrary.org/permissions
Rights-holder: Copyright held by individual article author(s).
Volume: 11
Item Size: 23.4M

Abstract: The two cultivated Canavalia (Adanson, 1763) species, Canavalia gladiata (N. J. von Jacquin, 1788) A. P. de Candolle, 1825 and Canavalia ensiformis (Linnaeus, 1753) A. P. de Candolle, 1825 are closely related based on morphological and molecular phylogenetic data. However, the similarities and differences in genome organization between them have not been evaluated at molecular cytogenetic level. Here, detailed karyotypes of both species were constructed using combined PI and DAPI (CPD) staining, rDNA-FISH and self-genomic in situ hybridization (sGISH). For further comparison, comparative genomic in situ hybridization (cGISH) and sequence analysis of 5S rDNA were applied. Their chromosomes were accurately identified by sGISH and rDNA-FISH signals. Both species had the karyotype formula 2n = 22 = 18m + 4m-SAT, but the karyotype of C. ensiformis was shorter and more asymmetric than that of C. gladiata. They displayed similar CPD bands at all 45S rDNA sites and centromeres. C. gladiata had ten centromeric 5S rDNA loci and two SC (secondary constriction)-associated 45S rDNA loci. C. ensiformis had nine centromeric and one interstitial 5S loci, two SC-associated and one proximal 45S loci. Their sGISH signal patterns displayed both basic similarities and distinct differences. Reciprocal cGISH generated prominent signals in all pericentromeric regions and 45S sites. There was lower level of sequence identity of the non-transcribed spacer between their 5S rDNA repeats. These data confirmed the evolutionary closeness between C. gladiata and C. ensiformis and demonstrated obvious differentiation between their genomes, and supported the opinion that C. ensiformis is more advanced in evolution than C. gladiata.
Addeddate: 2025-03-16 16:40:20
Bhl_virtual_titleid: 210879
Bhl_virtual_volume: v.11:no.4 (2017)
Call number: 10_3897_compcytogen_v11i4_13604
Call-number: 10_3897_compcytogen_v11i4_13604
Foldoutcount: 0
Genre: article
Identifier: molecularcytoge11shec
Identifier-ark: ark:/13960/s2x6j1wcr6p
Identifier-bib: 10_3897_compcytogen_v11i4_13604
Identifier-doi: 10.3897/compcytogen.v11i4.13604
Issue: 4
Ocr: tesseract 5.3.0-6-g76ae
Ocr_detected_lang: en
Ocr_detected_lang_conf: 1.0000
Ocr_detected_script: Latin
Ocr_detected_script_conf: 1.0000
Ocr_module_version: 0.0.21
Ocr_parameters: -l eng
Page_number_confidence: 81
Page_number_module_version: 1.0.5
Page_range: 579-600
Pages: 22
Pdf_degraded: invalid-jp2-headers
Pdf_module_version: 0.0.25
Possible copyright status: In copyright. Digitized with the permission of the rights holder.
Ppi: 300
Source: Comparative Cytogenetics 11(4)
Year: 2017